Extended Data Fig. 3: Measurement of the transposon on plasmid fraction before and after antibiotic treatment by plasmid extraction and transformation.
From: Intra- and interpopulation transposition of mobile genetic elements driven by antibiotic selection
Plasmids were extracted from the inoculation culture (a) and cultures after different antibiotic concentration treatment (b), and the fraction containing the resistance transposon was measured by qPCR (result shown in Fig. 2c) and then plasmids were transformed into E. coli DH5α. Next, qPCR was performed on the transformed culture to re-estimate the fraction of plasmids containing the resistant transposon. This result confirms the qPCR results shown in Fig. 2c. (mean ± S.D., n = 4). For the boxplot (a), the top whisker represents the maximum value, the top of the box represents the 75 percentile, the center line represents the median; the bottom of the box represents the 25th percentile, and the bottom whisker represents the minimum value.
