Extended Data Fig. 6: Larger field of views for microscopy images.
(a) Similar cellular morphology of compensated strains and natural isolates. The figure shows wider field of views for Fig. 2c. Images show pairs of compensated and natural strains that display similar morphological trait combinations (cell wall and nuclei are colored with green and red, respectively): (i) large cells with normal, wild-type-like elongation: xrs2-ev1, OS_1586 isolate from tree leaves, (ii) large round cells: vid22-ev2, OS_755 wine yeast isolate, (iii) small round cells: med1-ev4, OS_675 isolate from human blood. Scale bar represents 10 μm. (b) Synergistic epistasis underlying morphological changes in a compensated strain of Δrpb9. The figure shows wider field of view images for Fig. 5a. Images show 5 selected genotypes, including the wild-type (WT), two single mutants (Δrpb9 and Δwhi2) and a reconstructed double mutant (Δrpb9 + Δwhi2). The fifth genotype is the compensated strain of Δrpb9 (rpb9-ev2) that harbors the whi2S133* loss-of-function allele. Cell wall and nuclei are colored with green and blue, respectively.
