Extended Data Fig. 2: Results of principal component analysis on single-cell morphology.

(a) Cumulative variance of all single-cell morphological traits explained by the first 8 principal components in a principal component analysis (PCA). Note that PCA was performed on all genotypes, including wild-type and gene deletion ancestors. (bc) Contribution of specific morphological traits to the first 8 principal components. Panels (b) and (c) show the results of separate PCAs carried out for all strains and the subset of novel compensated strains (including the WT), respectively. Colors of the bars indicate the sign of the effects of specific traits on the given principal component (loading). Only traits providing the 8 largest contributions to the principal components are shown. Note that the traits contributing to PC1 to PC8 in panels (b) and (c) show substantial overlap with each other.