Extended Data Fig. 4: CYP4G19 modulates CSP production by affecting the biosynthesis of hydrocarbon precursors.
From: A single gene integrates sex and hormone regulators into sexual attractiveness
a, RNAi knockdown of CYP4G19 from N6 causes a depletion at the protein level (inset) and downregulation in cuticular hydrocarbons in adult females. The bands of tubulin were spliced from those of dsMuslta- and dsCYP4G19-injected groups, each with three lanes, on the same membrane. IS, internal standard of n-hexacosane. Similar results were obtained from six biological replicates. b, Effects of CYP4G19 knockdown on dimethyl alkane and methyl ketone production. n = 6 biological replicates. c, Tissue distribution of two other CYP gene expressions in adult females and the effect of their knockdown on CSP production. n = 4 (gene relative expression) and 6 (methyl ketone production) biological replicates. P values were determined by two-tailed unpaired t-test (b, c). Data in bar plots are mean ± s.e.m.
