Extended Data Fig. 5: JH signalling regulates the expression of CYP4PC1 but not CYP4G19.
From: A single gene integrates sex and hormone regulators into sexual attractiveness
a, Developmental similarity of Kr-h1 and CYP4PC1 expression across N6 and the first vitellogenic cycle. Inset of the right panel indicates a magnification of CYP4PC1 expression throughout N6. n = 4 biological replicates. b, c, Effect of JH III treatment in adult females on Kr-h1 expression in the antennae and abdominal integument (b), and CYP4G19 expression in the abdominal integument (c). n = 4 biological replicates. d, RNAi knockdown of Met or Kr-h1 in adult females is efficient in the abdominal integument. n = 4 biological replicates. e, Kr-h1 relative expression in the antennae from females with Kr-h1 knockdown and those with both dsKr-h1 and JH III treatments. n = 4 biological replicates. f, JH III application in adult males causes substantial transcription of CYP4PC1 in the antennae. The complete coding sequence of CYP4PC1 was detected in the antennae. P were determined by two-tailed unpaired t-test (b–d). Different letters indicate significant differences using one-way ANOVA (Tukey HSD multiple comparisons test, P < 0.05) (e). Data in a–e are mean ± s.e.m.
