Extended Data Fig. 6: Functional characterization of the FOXL2 enhancer.

Top panel, female-related ATAC peaks in the upstream of FOXL2. Middle panel, plasmid construction from FOXL2-peak1 to FOXL2-peak3 (right to left) used in dual-luciferase assays. Bottom panel, enhancer activities of peaks 1–3 as measured by luciferase assays. The peaks 1–3 showed significant enhancer activity compared to the empty vector pGL3-promoter (based on the two-sided t-test with n=3 biologically independent samples). For boxplot, centre line and box limits represent the median, upper and lower quartiles respectively, whereas whiskers are 1.5x interquartile ranges.