Extended Data Fig. 9: Stable Isotope Tracing of Juvenile Hormone (JH).
From: Convergent and complementary selection shaped gains and losses of eusociality in sweat bees
Absolute quantification of JH III in the brain (a) and bodies (d) of bumblebees treated with acetone or JH III-D3. Absolute quantification of labelled JH III in the brains (b) and bodies (e) of bees treated with acetone or JH III-D3. Labelled JH III-D3 levels are high after 24 hours, but decay significantly by 72 hours. (c) Labelled JH III-D3 applied to abdomens of bumblebees is detectable in brains 24 h later (acetone is control). n = 3 tissues/condition. (f) JH III-D3 accounts for nearly all the total JH III in bee bodies after 24 hours, indicating that the labelled compound is well-absorbed by the bee. n = 3 bumblebee workers for each experimental condition. Boxes show median, 25th and 75th percentiles. Whiskers show minimum and maximum values.
