Extended Data Fig. 7: Additional nanopore deletion and structural variant analysis, and Illumina exome enrichment characterisation.

(7a) Prominent human cancer-associated genes with detections within or adjacent to the gene detected (gnomAD database) detected in Moultrie Creek B nanopore shotgun water sample. Deletion location denoted by blue shading. Full details of all detected deletions can be found in Supplemental Table 5. (7b) Structural variant types identified in nanopore eDNA sequence data identified by the EPI2ME sniffles-based structural variant caller. (7c) Illumina exome enrichment versus shotgun nanopore sequencing data. Left: Total number of DNA bases mapped to the human genome (minimap2) for all exome samples, including negative field controls (Moultrie creek negative field control water eDNA and Rattlesnake Island site 2 sample 1 [no human site] sand eDNA). Right: Total number of DNA bases mapped to the human genome (minimap2) for all human exome eDNA samples, and the corresponding shotgun nanopore data (where the sample was sequenced with both approaches). Note, information is in bases not in reads because bases account for variable read length between nanopore and Illumina sequencing. For mapped read and mapped base information for all analysed samples see Supplemental Table 4.