Extended Data Fig. 8: Metagenomic analysis of nanopore data and qPCR-based green sea turtle species-specific qPCR-based quantification from room air eDNA samples.

(8a) Metagenomic taxonomy of microbial and human aligning reads from the high human site eDNA water sample (Moultrie Creek B), inclusion cut-off on tree, each branch contains at least 0.01% of reads. (8b) Metagenomic taxonomy of microbial and human aligning reads from the human footprint sand eDNA sample (human male footprint 4), inclusion cut-off on tree, each branch contains at least 0.01% of reads. (8c) qPCR-based species-specific quantification of green sea turtle (C. mydas) eDNA from room air samples. Absolute quantity (10E-3 pg/μl per reaction) of green sea turtle eDNA per sample. A 499 bp synthetic gene fragment was used for standard curve generation. Each qPCR reaction is a 10 μl reaction containing 1 μl of extracted eDNA template.