Extended Data Fig. 9: Green sea turtle genome coverage map from room air eDNA, and human qPCR assay cross-reactivity qPCR. | Nature Ecology & Evolution

Extended Data Fig. 9: Green sea turtle genome coverage map from room air eDNA, and human qPCR assay cross-reactivity qPCR.

From: Inadvertent human genomic bycatch and intentional capture raise beneficial applications and ethical concerns with environmental DNA

Extended Data Fig. 9: Green sea turtle genome coverage map from room air eDNA, and human qPCR assay cross-reactivity qPCR.

(9a) Green sea turtle (C. mydas) genome alignment map of the green turtle aligning reads (minimap2) from the 5 hr 30 min room air eDNA sample. (9b) Human eDNA qPCR assays cross-reactivity test with sea turtle (loggerhead and green) tissue samples. Absolute quantity of human genomic DNA present (ng/μl per reaction). Each qPCR reaction is a 10 μl reaction containing 1 μl of extracted genomic DNA template. Note that both assays were species-specific with no cross-reactivity (no amplification) in either sea turtle tissue sample. Loggerhead and green sea turtles can be added to the list of 27 other species (from mice to plants, see methods section) experimentally validated with which these human assays do not cross-react.

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