Extended Data Fig. 2: Abrogation of T6SS activity does not provide a fitness benefit to B. fragilis under laboratory conditions growing on polysaccharides as carbon source.
The growth of the producer and resistant strains in defined minimal media with different carbon sources (polysaccharide) is shown in liquid and solid (mono and coculture) growth in the different panels. For solid growth, B. fragilis producer strain with ErmR and resistant strain with TetR were grown on defined minimal media with different carbon sources separately (monoculture) and together in a 1:1 ratio (coculture). Each strain was quantified via calculating colony-forming units on BHIS agar plates supplemented with erythromycin or tetracycline. Carbon sources used in defined minimal media included potato starch (a), glycogen (b) and mucin (c). Two-sided Mann-Whitney tests showed no difference between the different strains at each timepoint. For liquid experiments, the results are from three biological replicates that each represent the mean of eight technical replicates. For solid growth, results are from six biological replicates from two independent experiments. Data shown represent mean values ± SD.
