Extended Data Fig. 4: Correlation of GC, GC3 and coding density with chromosome length in Lepidoptera superfamilies.

a–c, Proportional chromosome length against GC proportion (a), GC proportion at the third codon position (GC3) (b), density of coding sequence (c). Proportional chromosome length is chromosome length divided by genome size. A locally weighted smoothing line (‘LOESS’) is drawn between the autosomes of each species. The line is coloured green if the correlation was significant (Spearman’s rank, p < 0.05), or orange if it was non-significant (Supplementary Table 8). Only species with at least 10 autosomes are included and all autosomes that we inferred to have undergone fusion or fission events were removed. Only superfamilies represented by at least 5 superfamilies are shown. The Z chromosomes were not included in the correlation analysis and are indicated in red.