Extended Data Fig. 3: Phylogeny of independently evolved line PO-3, showing deep divergence between Small and Large isolates.

They do not share any mutations, indicating that the last common ancestor of these lineages was the genotype used to found the experiment, and these lineages have been coexisting for the full duration of the experiment. Here, the color represents the phenotype (Small or Large), and numbers PO-3-1, PO-3-2, and PO-3-3 represent three isolates of Small and Large yeast sampled from line PO-3.