Extended Data Fig. 6: Validation of the bHLH35 functions in anthocyanin accumulation in Arabidopsis thaliana and different patterns of anthocyanin accumulation in A. thaliana and Corydalis hemidicentra.
a, Stable overexpression of the orthologous AtbHLH35 (AT5G57150.4) in A. thaliana, scale bars = 1 cm. Transgenic plant were treated with normal ( ~ 150 μmol m−2s−1) or high light ( ~ 400 μmol m−2s−1) stress for seven days. b, Anthocyanin, chlorophyll and carotenoids concentrations for different transgenic lines. Data represent the mean ± SD from four biological replicates. P values are from two-sided Student’s t test. c, qRT-PCR for early (CHS and CHI), late (F3H, DFR and ANS) and final (UFGT) anthocyanin biosynthesis pathway genes in the rosette leaves of A. thaliana. Data represent the mean ± SD from three biological replicates. d, The proportion of anthocyanin categories. Anthocyanidins are differentially substituted on the anthocyanidin B-ring as follows: Cyanidin R1 = OH, R2 = H; Delphinidin R1 = R2 = OH; Malvidin R1 = R2 = OMe; Pelargonidin R1 = R2 = H; Peonidin R1 = OMe, R2 = H Petunidin R1 = OH, R2 = OMe. e, Absolute quantitation of anthocyanins in A. thaliana (n = 3) and C. hemidicentra (n = 4). Data represent the mean ± SD. P values are from two-sided Student’s t test.
