Fig. 4: Extended single-cell stage and organ profiling and optic lobe maturation.

a, UMAP of 19,029 cells from a 1-dph hatchling. b–d, A comparison of the optic lobe morphology by micro-CT segmentation and rendering of optic lobes 1 day after hatching (b), 40 days after hatching (c) and 80 days after hatching (d). e, A stacked bar plot of the number of cells of each cell type in each single-cell dataset. The inset shows an anterior view of the optic lobes at 1 dph with the minute optic nerve located ventrally (yellow) f,g, Heat maps showing expression levels of cholinergic (f) and glutamatergic (g) markers in adult and hatchling optic lobes.