Extended Data Fig. 6: Marked trade-offs between catalytic efficiency and periplasmic stability.
From: Cryptic phenotypic variation emerges rapidly during the adaptive evolution of a carbapenemase
(a) Catalytic efficiency shows a weak, positive, nonsignificant correlation with MIC increases (Pearson’s r = 0.37, P = 0.47). (b) Periplasmic stability exhibits a strong negative correlation with MIC increases (Pearson’s r = –0.93, P = 0.007). (c) Combining catalytic efficiency and periplasmic stability (as in ref. 83) still strongly negatively correlates with MIC increases (Pearson’s r = –0.91, P = 0.0109). Although higher stability should intuitively increase MIC by providing greater enzyme availability, this expectation only holds true in the absence of the activity-stability trade-offs observed here. Note these factors do not offset each other effectively because losses in stability are orders of magnitude greater than gains in catalytic efficiency.
