Extended Data Fig. 7: Antp P2 mKO crispants in B. anynana.

Two guide RNAs co-injected efficiently induced long deletions around the conserved sequences (Fig. 4d middle panel) of Antp P2 in vivo, confirmed in pooled injected eggs (upper panel). The WT bands and mutant bands are denoted by black and red arrowheads, respectively. Genomic DNA was extracted from right hindwings of eight mKO crispants, and subjected to PCR and gel electrophoresis to detect potential long deletions (middle panel). Three crispants showing clear long deletions were genotyped via Nanopore amplicon sequencing. A dotted line separates left and right sides of the same individual. Purple arrowheads denote the wings used for genotyping, and red arrowheads denote guide RNA cut sites. Sequence coverage (Cov.) across the amplicon and major KO alleles are shown (lower panel).