Extended Data Fig. 8: Antp P1 mKO crispants in B. anynana.

Two guide RNAs co-injected efficiently induced long deletions around the conserved sequences (Fig. 4d right panel) of Antp P1 in vivo, confirmed in pooled injected eggs (top panel). The WT bands and mutant bands are denoted by black and red arrowheads, respectively. A dotted line separates left and right sides of the same individual. Purple arrowheads denote mutant phenotypes.