Extended Data Fig. 9: An Antp P1 mutant line in B. anynana.

A mutant line carrying a 252 bp deletion around the conserved region (Fig. 4d right panel) of Antp P1 was generated (Methods). The gel image shows clear differentiation of PCR bands across WT, mutant heterozygotes, and mutant homozygotes. The WT bands and mutant bands are denoted by black and red arrowheads, respectively. Representative wings for each genotype in both seasonal forms are shown. A dotted line separates dorsal (left) and ventral (right) sides of the same individual.