Extended Data Fig. 1: Temperature-shift experiments identified a prolonged temperature sensitivity window. | Nature Ecology & Evolution

Extended Data Fig. 1: Temperature-shift experiments identified a prolonged temperature sensitivity window.

From: A novel Hox gene promoter fuels the evolution of adaptive phenotypic plasticity in wing eyespots of satyrid butterflies

Extended Data Fig. 1

Shift schemes in refined temperature-shift experiments are illustrated (left panel). See Methods and Supplementary Table 1 for detailed methods and statistics of the temperature-shift experiments. Ventral Cu1 eyespot areas (middle panel), and temperature sensitivities (right panel, only across the six non-overlapping windows) were quantified and compared across the schemes in a one-way ANCOVA (Methods). n = 35 (WS), 20 (L5-1), 20 (L5-2), 12 (L5-3), 24 (Wr), 23 (PP), 22 (P15), 23 (Wr+PP), 23 (Wr+PP + P15), 24 (L5), 27 (DS). Black dots with error bars represent mean values ± 95CI. Eyespot areas from shift schemes with the same letter are not significantly different from each other, as determined by Tukey’s test. WS, wet season; DS, dry season; L5, 5th instar larva; Wr, wanderer, PP, prepupa; P, pupa.

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