Extended Data Fig. 6: Chemolithoautotrophic gene quantification.
From: Distinct contributions of suspended and sinking prokaryotes to mesopelagic carbon budget
Each boxplot includes data pooled from all sampled stations and depths (n = 20). Overlayed boxplots indicate the interquartile range (boxes), median (central line), and whiskers extending to 1.5×IQR. Statistically significant differences are indicated with asterisks (two-sided Wilcoxon-Mann-Whitney statistical test: **** p ≤ 0.0001; *** p < 0.001; * p < 0.05). (A) Ratio of total chemolithoautotrophic communities (gene copy numbers calculated as the sum of the marker genes accA, cbbL and cbbM) in the entire prokaryotic community (sum of bacterial and archaeal 16S gene copy numbers). qPCR gene ratio for AOA (ratio of amoA gene / 16S prokaryotes gene) and AOB (ratio of amoB gene / 16S prokaryotes gene) in (B) the “Suspended” fraction (that is, free-living and prokaryotes attached to suspended particles) and in (C) the “Sinking” fraction (that is, prokaryotes attached to sinking particles).
