Extended Data Fig. 3: Illustration of the sampling methods employed during the APERO cruise to study prokaryotic fraction.
From: Distinct contributions of suspended and sinking prokaryotes to mesopelagic carbon budget

“Suspended” prokaryotes (that is, free-living and attached to suspended particles) and “sinking” prokaryotes (that is, attached to sinking particles) were sampled by Niskin and sediment traps respectively. The investigation of prokaryotic communities included PHP (3H-leucine) and DCF (14C-bicarbonate) measurements, prokaryotic cell abundance and gene quantification (qPCR) of chemoautotrophy in all PSSs. A single cell nanoSIMS analysis (13C-bicarbonate and 15N-ammonium) was added in PSS3 at 500 m. CTD rosette illustration reproduced with permission from ref. 72.