Extended Data Fig. 8: Ectopic expression of Itgav and Itgb3 in the liver could re-direct the delivery of 6R-LNP in vivo.
(a) Experiment design of the hepatic overexpression of Itgav and/or Itgb3 in mouse liver using 3R-LNP. (b) qPCR analysis of the overexpression of Itgav and/or Itgb3 in the liver under indicated conditions. n = 3 animals for each condition. (c) Flow cytometry analysis of the overexpression of Itgav and/or Itgb3 in the liver under indicated conditions. n = 3 animals for each condition. (d) Quantification of Itgav+Itgb3+ (that is, VtnR + ) cell population in liver after overexpression of Itgav and/or Itgb3 as indicated. n = 3 animals for each condition. (e) The workflow of Luc mRNA delivery using 6R-LNP. (f) Ex vivo organ luminescence images showing the expression level of luciferase in the liver, lung, spleen, heart, and kidney, respectively, of mice with ectopic hepatic overexpression of Itgav and/or Itgb3 as indicated. n = 3 animals for each condition. (g) Quantification of luminescence of the liver (left) and lung (right), respectively, of mice with ectopic hepatic overexpression of Itgav and/or Itgb3 as indicated, after Luc mRNA delivery by 6R-LNP. n = 3 animals for each condition. (h) Stacked bar chart showing the relative expression of luciferase in the liver, lung, and spleen, respectively, of C57/BL6 wild-type mice with ectopic hepatic overexpression of Itgav and/or Itgb3 as indicated, after Luc mRNA delivery by 6R-LNP. n = 3 animals for each condition. One-way analysis of variance (ANOVA) and Dunnett’s multiple comparisons test were used for statistical analysis. ns: p > 0.05. All data were plotted as the mean ± s.d. Schematics in a and e created using BioRender.com.
