Extended Data Fig. 10: POST-LNP could deliver mRNA into parenchymal cells in target organs.
(a) UMAP plotting showing cell identity annotations in the lung. Cell types are colored according to the dot representative of each cluster. (b) Featured UMAP plotting showing single-cell distribution of Luc mRNA in the lung after delivery by lung-specific 6R-LNP. (c) UMAP plotting showing cell identity annotations in the spleen. (d) Featured UMAP plotting showing single-cell distribution of Luc mRNA in the spleen after delivery by spleen-specific 6D-LNP. (e) Percentage of cells with exogenously delivered Luc mRNA by 6R-LNP in the lung. (f) Percentage of cells with exogenously delivered Luc mRNA by 6D-LNP in the spleen. n = 4 animals for each condition. (g) Quantification of the percentage of EGFP+ cells in the fibroblasts, alveolar type I cells, alveolar type II cells, and basal progenitor cells, respectively. (h) Quantification of the percentage of EGFP+ cells in the B cells, macrophage, and T cells respectively. (i) Quantification of the percentage of EGFP+ cells in the trophoblast cells. (j) Quantification of the percentage of EGFP+ cells in the long-term hematopoietic stem cells (LT-HSC) and multi-lineage reconstituting CD150 + LT-HSC. (k) Quantification of the percentage of EGFP+ cells in the adipocyte progenitor cells. (l) Quantification of the percentage of EGFP+ cells in the spermatogonia cells. n = 3 animals for each condition. P-values were calculated using unpaired Student’s t-test. All data were plotted as the mean ± s.d.
