Extended Data Fig. 3: Vtn adsorption selectively facilitates cellular uptake of 6R-LNP in vitro.
(a) The schematic of the experiment procedures. (b) Representative confocal images showing cellular uptake of LNPs that are labeled with Cy5-DSPE. Scale bar: 50 μm. (c) Quantification of cells containing Cy5-LNPs under indicated conditions. n = 5 confocal images analyzed for each condition. n = 3 independent experiments for each condition. (d) Quantification of luciferase expression following delivery of luciferase mRNA using LNPs with or without adsorption of Vtn protein. n = 3 independent experiments for each condition. One-way analysis of variance (ANOVA) and Dunnett’s multiple comparisons test were used for statistical analysis. All data were plotted as the mean ± s.d. Schematic in a created using BioRender.com.
