Extended Data Fig. 5: Intravascular behaviour of human tumour cells confirms link between viscosity and circulation and arrest patterns.
From: Cell viscosity influences haematogenous dissemination and metastatic extravasation of tumour cells
a, Ratio of elasticity of M229 R / S and CTC- / CPP-45 tumour cell line pairs normalized to M229 R and CTC-45 respectively (N = 6 (M229 R, M229 S) and 3 (CTC-45, CPP-45), n (cells) = 24 (M229 R), 27 (M229 S), 16 (CTC-45), 22 (CPP-45)) (Two-tailed Mann-Whitney (M229 R vs M229 S); Two-tailed Welch’s t-test (CTC-45 vs CPP-45)). b, Ratio of viscosity of M229 R / S and CTC- / CPP-45 tumour cell line pairs normalized to M229 R and CTC-45 respectively (N = 6 (M229 R, M229 S) and 3 (CTC-45, CPP-45), n (cells) = 24 (M229 R), 27 (M229 S), 16 (CTC-45), 22 (CPP-45)) (Two-tailed Mann-Whitney). c, Tumour cells arrested in ZF embryos 5 mpi. Yellow arrows indicate tumour cells arrested in small vessels. d, Heatmaps displaying hotspots of arrest 5 mpi of tumour cells in ZF embryo (N = 2 (MCF-7, MDA-MB-231, M229 R, M229 S, CTC-45 and CPP-45), n (embryos) = 11 (MCF-7), 18 (MDA-MB-231), 12 (M229 R and M229 S), 13 (CTC-45) and 15 (CPP-45)). e, Fold changes in arrest patterns in ZF embryo 5 mpi and viscoelastic properties for paired tumour cell lines. f, Correlation test of fold change in ratio of arrest occurring in small vessels and fold change in ratio of elasticity (Extended Data Figs. 3f, 4h and 5e) (Spearman r). Data are presented as mean +/- standard deviation in a-b.
