Extended Data Fig. 7: Endothelial remodeling dynamics are affected by tumour cell viscosity and don’t require of tumour cells that they undergo deformation.
From: Cell viscosity influences haematogenous dissemination and metastatic extravasation of tumour cells
a, Ratio of intravascular cells 3 hours post-injection of mechanically altered tumour cells. (N = 4 (siCTRL); 2 (siMYH9, siVIM, siCAV1, siLMNA), n (embryos) = 45 (siCTRL); 12 (siMYH9); 24 (siVIM); 23 (siCAV1); 17 (siLMNA)) (Kruskal-Wallis followed by original FDR method of Benjamini and Hochberg). b, Ratio of pocketed cells in large vessels of the ZF embryo 3 hpi of mechanically-altered tumour cells. (N = 4 (siCTRL); 2 (siMYH9, siVIM, siCAV1, siLMNA), n (embryos) = 44 (siCTRL); 11 (siMYH9); 22 (siVIM); 23 (siCAV1); 16 (siLMNA)) (Kruskal-Wallis followed by original FDR method of Benjamini and Hochberg). c, Ratio of pocketed or extravasated cells 3 hpi for single cells vs grouped cells. (N = 4, n (cells) = 42 (single) and 32 (grouped)) (Two-tailed Mann-Whitney). d, D2A1 tumour cells pocketed in the DA, AVJ, CV, ISVs and CPCs 3 hpi. Yellow stars indicate tumour cell-containing endothelial pockets. e, D2A1 tumour cells extravasated between the CV and the CPCs. Yellow stars indicate the location of the cells in the extravascular space between the CV and the CPCs. f, D2A1 tumour cells extravasated around ISVs 3 hpi. Yellow star indicates extravascular location of the tumour cell. g, Ratio of early extravasation events in different vascular subregions of the ZF embryo 3 hpi of siCTRL-treated D2A1 tumour cells. (N = 4, n (embryos) = 26 (DA), 39 (AVJ), 27 (ISVs) and 34 (CV+CPCs)) (Kruskal-Wallis followed by original FDR method of Benjamini and Hochberg). h, Graphical summary of the indirect impact of cell viscosity on tumour cell extravasation. As established previously, the constraining vessels in which early completed extravasation events occur are more accessible to low viscosity tumour cells. i, Pocketed D2A1 tumour cell 3 hpi and corresponding 3D renders. Yellow star indicates tumour cell-containing endothelial pocket. j-l, Normalized volume, elongation and flatness of intravascular, pocketed and extravasated D2A1 tumour cells 3 hpi. (N = 2, n (cells) = 78 (intravascular), 12 (pocketed) and 15 (extravasated)) (Ordinary one-way ANOVA followed by original FDR method of Benjamini and Hochberg). Data are presented as mean +/- standard deviation in a-c, g and j-l.
