Extended Data Fig. 10: Pan-organ assessment of the lasting impact of tumour cell viscoelasticity on metastatic outgrowth.
From: Cell viscosity influences haematogenous dissemination and metastatic extravasation of tumour cells
a, Pan-organ bioluminescence imaging-based assay for metastatic outgrowth assessment 15 dpi. b-l, Bioluminescence imaging and quantification of organs displaying bioluminescence total flux superior to the average of the siVIM group 15 dpi (intracardiac) of siRNA-treated D2A1 tumour cells. N (Experiments) = 3 for all organs probed. n (number of organs) = 9 (siCTRL), 12 (siVIM), 9 (siMYH9) for brains, 8 (siCTRL), 11 (siVIM), 9 (siMYH9) for livers, 9 (siCTRL), 13 (siVIM), 8 (siMYH9) for guts, 8 (siCTRL), 13 (siVIM), 8 (siMYH9) for pairs of kidneys, 8 (siCTRL), 10 (siVIM), 8 (siMYH9) for ovaries / uterus and 8 (siCTRL), 13 (siVIM), 8 (siMYH9) for spleens. Chi-square tests were performed in c, e, g, i, k and m). 9 (siCTRL), 13 (siVIM) and 9 (siMYH9) female 10-week-old BALB/c mice were used in this experiment.
