Extended Data Fig. 2: IFI16 inhibits IAV viral replication.
a, A549 cells were transfected with IFI16-Flag plasmids or empty control for 24 h and then infected with PR8 virus at 2.0 MOI. mRNA and vRNA expression of NP and M genes at 6, 12 and 18 hpi were determined by RT-qPCR. Data are presented as means ± SEMs from three independent experiments. b, HEK293 cells were transfected with IFI16-Flag plasmids or empty control for 24 h and then infected with PR8 virus at 2.0 MOI. NP protein expression at 0, 6, 12 and 18 hpi was determined by Western blotting. β-Actin detection was used as loading control. (c) and (d), A549 cells were transfected with IFI16-targeting siRNA and negative control (NC) siRNA for 24 h, followed by infection with PR8 virus (MOI = 1). c, NP and M1 protein expression were determined by Western blotting at 0, 12, 18 and 24 hpi. β-Actin detection was used as loading control. d, Viral titers were determined by TCID50 assay at the 24 hpi. Data presented as means ± SD from three independent experiments. (b) and (c), Data are representative of three independent experiments. Statistical significance in (a) and (d) was determined by unpaired two-tailed Student’s t-test. ns = non-significant.
