Extended Data Fig. 3: IFI16 enhances the activation of IFN-I pathway during IAV infection.
a to c, Serum-starved A549cells were transfected with IFI16-encoding plasmids or negative control, and then infected with 1.0 MOI of PR8 virus. Expression of IFN-β (a), ISG15 (b), IL-6 (c) at the indicated time points was determined by RT-qPCR. d to f, mRNA expression of IFN-α4 (d), IRF7 (e) and CXCL5 (f) in PR8 virus-infected IFI16+/+ and IFI16−/− A549 cells at 0, 6, and 12 hpi was determined by RT-qPCR. (g) Immunoblot analysis of RIG-I-triggered downstream signaling pathway in IFI16+/+ and IFI16−/− A549 cells after stimulation with 5′ppp-RNA for the indicated duration. h, IFI16+/+ and IFI16−/− A549 cells transfected with IFI16-Flag expression vectors or empty control for 24 h and then infected with PR8 virus at 1.0 MOI. RIG-I-triggered downstream signaling pathway at 0, 4, 8 and 12 hpi was assessed with indicated antibodies. a to f, Data presented as means ± SD from three independent experiments. g to h, Data are representative of three independent experiments. Statistical significance in (a) to (f) was determined by unpaired two-tailed Student’s t-test. ns = non-significant.
