Extended Data Fig. 3: PDIM inhibits NADPH oxidase recruitment to Mtb phagosomes.
From: Mycobacterium tuberculosis virulence lipid PDIM inhibits autophagy in mice
(a-d) WT BMDMs were infected with GFP-expressing Mtb strains for 4 h. Single cell bacterial suspension was generated by (a-b) slow speed spin or (c−d) passage through a 5μm filter. (a, c-d) IF maximum-intensity projection images of (a) p40phox, (c) LC3 and p40phox, and (d) LC3 and GAL3. Scale bar, 10 μm for leftmost panels and 2.5μm for all zoomed images. Boxed areas in the merged image are shown in higher magnification in the rightmost panels. (b) Automated image analysis was used to quantify the MFI of p40phox colocalized with individual bacilli from 5 fields. Data consists of more than 100 bacilli per group. Data are means ± SEM from one representative experiment of three. Unpaired two-tailed t test. Automated quantification of MFI of panels c and d are shown in Fig. 2g–j.
