Extended Data Fig. 4: The GMT island in V. parahaemolyticus 04.2548 is a functional mobile element.
From: Gamma-Mobile-Trio systems are mobile elements rich in bacterial defensive and offensive tools
(a) Schematic representation of the GMT island in V. parahaemolyticus 04.2548 (cyan rectangle). A predicted intragenic (tRNA-Gly), inverted repeat-containing (pink and purple-colored sequences) naïve insertion site identified in V. parahaemolyticus 20140829008-1 is shown below. Inverted repeat sequences flanking the GMT island are denoted. GenBank accession numbers are provided. (b) Agarose gel electrophoresis analysis of the indicated amplicons. The total DNA isolated from wild-type V. parahaemolyticus 04.2548 cells conjugated with an empty plasmid (pEmpty), a plasmid containing a predicted naïve insertion site for VPaI-6 (pNISVPaI-6), or a plasmid containing a predicted naïve insertion site for the 04.2548 GMT island (pNIS04.2548), was used as a template. Arrows denote the positions of primers used for each amplicon; the expected amplicon size is denoted in gray. cat, chloramphenicol resistance gene found on the plasmids. A representative result out of at least three independent experiments is shown.
