Extended Data Fig. 4: Relative cdGMP levels of strains used in the study.

Relative values of cdGMP were measured using a previously published fluorescence-based reporter (GFP under the control of the cdrA promoter by Rybtke et. al.⁹²). See Methods section for details. Each data point represents an independent biological replicate (n = 3); horizontal black lines mark their mean. Statistics: 1-way ANOVA with Tukey HSD multiple comparison test, with asterisks showing significant differences relative to WT (* p < 0.05, *** p < 0.001). Due to the large difference in magnitude of the cdGMP levels for strains with the ∆wspF deletion, the comparisons were made in two parts (part 1: WT vs ∆algR or ∆bifA; part 2: WT vs. ∆wspF or ∆wspF∆pel∆psl). The data underlying this figure and information on statistical analyses can be found in the source data available with this manuscript (see Data Availability session).