Extended Data Fig. 9: Matrix production mediates tolerance to CIP in high cdGMP strains.

A. Validation of the growth defects caused by cdGMP-mediated matrix overproduction at the mucosal surface using CFUs. Each data point represents an independent biological replicate (that is, one transwell, n = 3); the horizontal black lines mark their mean. B. Tolerance to CIP at the mucosal surface measured by CFUs for different strains used in our study. For each strain, tolerance is calculated based on total CFUs recovered before and after CIP treatment. Each data point represents an independent biological replicate (that is, one transwell, n = 3); the horizontal black lines mark their mean. Statistics: panel A, 1-way ANOVA with Tukey HSD multiple comparison test, with asterisks showing significant differences relative to WT (** p < 0.01, *** p < 0.001, n.s. p > 0.05); panel B, 1-way ANOVA with Tukey HSD multiple comparison test, with asterisks displaying significant differences for highlighted comparisons (* p < 0.05, n.s. p > 0.05). In panel B, statistical analyses were run in two parts (part 1: WT vs ∆bifA or ∆wspF∆pel∆psl; part 2: ∆wspF vs ∆wspF∆pel∆psl or WT). All experiments were performed with CF HBE cells. Abbreviations: CFUs, colony-forming units; CIP, ciprofloxacin. The data underlying this figure and information on statistical analyses can be found in the source data available with this manuscript (see Data Availability session).