Extended Data Fig. 2: Functional annotation of Tn-seq data using Database for Annotation, Visualization and Integrated Discovery (DAVID). | Nature Microbiology

Extended Data Fig. 2: Functional annotation of Tn-seq data using Database for Annotation, Visualization and Integrated Discovery (DAVID).

From: Pseudomonas aeruginosa faces a fitness trade-off between mucosal colonization and antibiotic tolerance during airway infection

Extended Data Fig. 2

We used the list of significant genes found in comparison #1 of our Tn-seq analysis (see Supplementary Table 1) as input for DAVID and used the web tool to calculate the fold enrichment of Gene Ontology (GOTERM_BP_DIRECT) terms. Disruption of processes found on top leads to decreased fitness (magenta), while disruptions of processes found on the bottom lead to increased fitness (green). The results of the same analysis but using KEGG Pathways can be found in Fig. 1B. For full parameters calculated in the analyses (for example, gene counts, % ID, p-values) displayed here and in Fig. 1B, see Supplementary Table 2. The data underlying this figure can be found in the source data available with this manuscript (see Data Availability session).

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