Fig. 2: Growth properties of Mtb and M. smegmatis.
a,b, Distributions of Lb, Ld, Td and elongation speed \(\left(\frac{{{\rm{d}}L}}{{{\rm{d}}t}}\right)\) are shown for both Mtb (n = 363; video set A) (a) and M. smegmatis (n = 391) (b). The coefficient of variation is shown in the upper right corner of each plot. The centre line of each box-and-whisker plot indicates the median. The left whisker extends from the minimum value to the lower quartile, and the right whisker extends from the upper quartile to the maximum value. The M. smegmatis data are from a previous study25. c,d, Growth property distributions are compared between acc and alt cells in Mtb (n of acc = 173, n of alt = 130) (c) and M. smegmatis (n of acc = 193, n of alt = 188) (d). Lb, Ld, elongation speed, growth amount and Td are compared between acc and alt, respectively. Horizontal lines mark the median value for each sample. The M. smegmatis data are from a previous study25. P values were calculated using the two-sided, two-tailed Mann–Whitney test. In Mtb, the P values for each comparison are 0.024, 0.66, 0.39, 0.27 and 0.80, respectively (c). The P values for M. smegmatis are 8.29 × 10−23, 6.66 × 10−20, 2.20 × 10−9, 0.0004 and 0.070, respectively (d). Mann–Whitney U = 9,542, 10,908, 10,592, 10,419 and 11,050 in Mtb and 7,576, 8,326, 11,712, 14,335 and 16,207 in M. smegmatis, respectively. e,f, Distributions of division asymmetry in Mtb, M. smegmatis and E. coli. The distributions of division asymmetry in Mtb (e) and M. smegmatis (f) are calculated using the equation Lb of alt cell (daughter cell)/Ld of mother cell. The division symmetry of E. coli is calculated using the equation Lb of daughter cell/Ld of mother cell (e). The M. smegmatis and E. coli data are from previous studies25,83. The dashed lines at 0.5 indicate perfect symmetry. The CVs of Mtb, E. coli and M. smegmatis are 12%, 6% and 22%, respectively. *P < 0.05; ***P < 0.001; ****P < 0.0001. NS, not significant.
