Extended Data Fig. 1: Single-cell traces of SSB-GFP localization (movie set A).

The distance between the x-axis and the black circle indicates cell length. Yellow circles indicate SSB-GFP foci. a-c, Cells with a cell cycle comprised of B-C-D periods. The duration of each period can vary between single cells. d,e, Cells with cell cycle comprised of B-C-D-E periods. Cells with an E period initiate another round of DNA replication before division. The daughter cells of this type of cell lack the B period and enters C directly. f, Example cell with a cell cycle comprising a C and D period. This type of cell cycle occurs in cells whose mother begins replication before division (E period). In cases where foci disappeared for a frame or two due to focus issues and then reappeared, we assumed replication continued until the last foci disappeared. g, Proportional distances between new pole or old pole and SSB foci. The left cartoon illustrates how the proportional distance between SSB and new pole or old pole is calculated. The green circle indicates the location of SSB foci. In each cell, the distance was measured when either one or two SSB foci were present. To calculate the proportional distance, the average distance from either the new or old pole was divided by the length of the cell (for cells with one SSB focus: proportional distance between new pole and SSB = a⁄b; for cells with two SSB foci: proportional distance between new pole and SSB = a⁄c, between old pole and near SSB = b⁄c). On the right, the circles represent the proportional distance between SSB foci and the indicated pole (cell numbers with one SSB focus = 363, cell numbers with two SSB foci = 139). The red horizontal lines mark the median value. Each dot represents a single cell from three biological replicates. P-value was calculated using a two-tailed Mann-Whitney test.