Extended Data Fig. 6: Effect of A. radioresistens removal on proliferation and cellular populations in colonic crypts.
a, FACS plots showing GFP+ cell populations isolated from the colon of Axin2-2A-EGFP reporter mice after vehicle or Ar-φ exposures (n = 6 per group). Top panel shows gating strategy for Axin2+ cells. b, Quantification of Lgr5+ cells after Ar-φ therapy in Lgr5-EGFP-IRES-creERT2 mice (Veh, n = 5 and Ar-φ, n = 6). c, FACS plots showing GFP+ cell populations isolated from the colon of Lgr5-EGFP-IRES-creERT2 reporter mice after vehicle or Ar-φ exposures (Veh, n = 5 and Ar-φ, n = 6). Top panel shows gating strategy for Lgr5+ cells. d, Representative images of EdU staining (DAPI staining, blue; EdU staining, green) of colons of mice exposed to vehicle or Ar-φ. White arrows, EdU-positive cells. Scale bar, 50 μm. e, Quantification of EdU-incorporating cells in the colons of vehicle or Ar-φ exposed mice (n = 5 per group). Data are reported as means ± SEMs. *P calculated using the Student’s t test (one-sided) and mentioned wherever it is <0.05.
