Extended Data Fig. 9: A. radioresistens modulates AhR-signaling in colonic stem cells.
a,b, Expressions of Wnt-2b (a) and Wnt-9a (b) mRNAs in the proximal colons of mice exposed to vehicle or Ar-φ (n = 6 per group). c, Expression of AhR mRNA in the proximal colons of AhRfl/fl (n = 6) versus Villin-cre-AhRfl/fl mice (n = 7). d, Representative images of EdU stained intestinal organoids from Villin-cre-AhRfl/fl mice exposed to OMVs from A. radioresistens, ∆trpC A. radioresistens or IAA for 24 hours. Scale bar, 50 μm. e, Quantification of EdU-incorporating cells in the organoids. Number of organoids assessed, Veh, n = 15, A. radioresistens, n = 13, ∆trpC A. radioresistens, n = 14 and 1 mM IAA, n = 14. f, Gain in body weights in Lgr5-EGFP-IRES-creERT2-AhRfl/fl-APCfl/fl mice exposed to vehicle or Ar-φ (n = 8 per group). g, Occult blood score in Lgr5-EGFP-IRES-creERT2-AhRfl/fl-APCfl/fl mice exposed to vehicle or Ar-φ (n = 8 per group). h, Quantification of microadenomas in vehicle treated Lgr5-EGFP-IRES-creERT2-APCfl/fl mice (n = 14) versus Lgr5-EGFP-IRES-creERT2-AhRfl/fl-APCfl/fl mice (n = 8). Data are reported as means ± SEMs. *P calculated using either Student’s t test (one-sided) or ANOVA with Tukey’s multiple comparisons test and mentioned wherever it is <0.05.
