Extended Data Fig. 3: A. radioresistens crosstalk with the colonic stem cells.
a, Representative images of colonoids from LGR5-2A-creERT2-R26-tdTomato mice exposed to A. radioresistens or ∆trpC A. radioresistens OMVs or IAA for 24 hours. Colonoids were treated with 4-Hydroxytamoxifen, 10 hours before experimental end point (blue, DAPI staining; red, Tomato). b, The percentage of Tomato positive cells in the colonoids exposed to A. radioresistens or ∆trpC A. radioresistens OMVs or IAA. Number of organoids assessed Veh (n = 13), A. radioresistens (n = 10), ∆trpC A. radioresistens (n = 10) and 1 mM IAA (n = 10). c, Representative images of organoids from Lgr5-EGFP-IRES-creERT2 mice showing colocalization of DiD-labelled A. radioresistens-OMVs (red) and GFP+ve LGR5 stem cells (green). d, Expression of Ascl2 mRNAs in organoids exposed to OMVs from A. radioresistens, ∆trpC A. radioresistens mutant or IAA for 24 hours. (n = 5 per group) Data are reported as means ± SEMs. *P calculated using ANOVA with Tukey’s multiple comparisons test and mentioned wherever it is <0.05.
