Extended Data Fig. 5: Dead-live staining shows neither bias towards FlagellaON cells nor for T3SSOFF cells during plant growth. | Nature Microbiology

Extended Data Fig. 5: Dead-live staining shows neither bias towards FlagellaON cells nor for T3SSOFF cells during plant growth.

From: Pseudomonas syringae subpopulations cooperate by coordinating flagellar and type III secretion spatiotemporal dynamics to facilitate plant infection

Extended Data Fig. 5: Dead-live staining shows neither bias towards FlagellaON cells nor for T3SSOFF cells during plant growth.

Graph shows the dead/live ratio for bacteria either expressing or not fliC::GFP3, hrpL::GFP3, hopAB1:GFP3 or hrcU::GFP3 during plant growth. Apoplast-extracted bacteria at 4 dpi in bean leaves were stained with a solution of Propidium iodide (PI). Data were obtained by flow cytometry analysis (100,000 events/ per replicate) and GFP levels were used to differentiate between ON and OFF cells using the non-fluorescent wild type strain as reference, as indicated before. Fluorescence of PI was used to differentiate dead and alive bacteria comparing to the non-fluorescent wild type. Each dot represents biological replicate in two different experiments (Rep 1 and Rep 2).

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