Extended Data Fig. 2: Flagella expression is heterogeneous on plant surface. | Nature Microbiology

Extended Data Fig. 2: Flagella expression is heterogeneous on plant surface.

From: Pseudomonas syringae subpopulations cooperate by coordinating flagellar and type III secretion spatiotemporal dynamics to facilitate plant infection

Extended Data Fig. 2: Flagella expression is heterogeneous on plant surface.

Selected images of the hopAB1::GFP3 fliC::tdT eCFP strain on the plant surface at 6 h post inoculation (hpi) by dipping the leaf into a 5 × 107 CFU/ml bacterial suspension. tdTomato panel shows the fluorescence of tdTomato associated to fliC expression, and eCFP panel shows the fluorescence of eCFP as constitutive expression reporter, using grey scale in both cases to improve contrast. The GFP panel, corresponding to hopAB1 expression, is not shown since no fluorescence was detected. Scale bars correspond to 20 μm. Contrast and brightness were adjusted to improve visualization but were kept constant across panels. Circles highlight bacteria detected in the eCFP panel without expression in the tdTomato channel (FlagellaOFF). Right panels correspond to the magnification of the area selected in rectangles. Images are representative of 3 independent experiments.

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