Extended Data Fig. 5: Simulation experiments to test bioluminescence of EcN-Ara-Lux.
(a) Schematic diagram of the arabinose-sensing genetic circuit. (b) Measurement of the bioluminescence densities of EcN-Ara-Lux when sensing different arabinose concentrations. (c) Measurement of the bioluminescence changes of EcN-Ara-Lux when sensing 10 mM arabinose within 4 h. (d) Imaging of EcN-Ara-Lux in mice model within 4 hours after the administration of strains and arabinose. (e), (f) Photocurrent measurement from the EcN-Ara-Lux when responding to different concentrations of arabinose. (g) Monitoring of arabinose distribution via the optoelectronic capsule while passing through an intestinal tissue segment containing evenly distributed EcN-Ara-Lux. (h) Imaging of the real pig intestinal section in (g). The capsule was pulled from the ‘Start’ point to the ‘End’ point slowly. (i) Changes of photocurrents along with the optoelectronic capsule passing through the intestinal section. (j) Schematic diagram of monitoring the arabinose-sensing EcN via optoelectronic capsule in the surgery-exposed pig colon sections. (k), (l) Comparison of the photocurrents measured from the EcN-Ara-Lux when adding arabinose or not. All the measurements in the above experiments were taken from distinct biological replicates. Data are presented as the mean ± SD. n = 3. Significance was determined by two-way ANOVA with Bonferroni’s post-test for (l) Source data. Panel j created with BioRender.com.
