Extended Data Fig. 3: FITC-labeled-gH/gL co-localizes with DSC2 expressed on TW03 cells.
From: Desmocollin 2 is a dominant entry receptor for Epstein–Barr virus infection of epithelial cells
(a) Validation experiments for the specificity of the rabbit anti-DSC2 antibody (10809-R004, Sino Biological) for immunofluorescence assay. WT and DSC2 knockout AGS single-cell clones (AGS-sgDSC2-c1) were stained with rabbit anti-DSC2 antibody and Alexa 488-conjugated secondary antibody, and imaged as green. Nuclei were stained with DAPI (blue). Scale bars,10 μm. (b) TW03 cells were incubated with 200 nM biotin-labeled gH/gL at 4 °C for 1 h. biotin-labeled gH/gL was stained with streptavidin-FITC and imaged as green. DSC2 was stained with rabbit anti-DSC2 antibody (10809-R004, Sino Biological) and Alexa 594-conjugated secondary antibody and imaged as red. Nuclei were stained with DAPI (blue). Scale bars,10 μm. Enlarged images are shown on the right. Data are representative of two (a, b) independent experiments.
