Extended Data Fig. 1: Flow cytometry gating for H5 TX/24 specific B cells. | Nature Microbiology

Extended Data Fig. 1: Flow cytometry gating for H5 TX/24 specific B cells.

From: Cross-neutralizing and potent human monoclonal antibodies against historical and emerging H5Nx influenza viruses

Extended Data Fig. 1: Flow cytometry gating for H5 TX/24 specific B cells.

Representative flow plots for isolation of H5 TX/24 + B cells. PBMCs isolated at peak time points were stained with antibodies to detect B cell surface markers and fluorescently labeled HA probes. CD19 + CD20+ IgG+ or IgA+ were gated and reactivity of B cells to the ectodomain of H5 Indo/05 and H5 TX/24, but not H5 Indo/05 stabilized stem, were single cell sorted.

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