Extended Data Fig. 2: Linoleic acid exports from Mtb-infected macrophages through ATP-binding cassette transporter G1 (ABCG1).
(a-g) qPCR analysis of Fabp4 (a), Fabp5 (b), Fatp1 (c), Fatp2 (d), Obp2a (e), Abca1 (f) and Abcg1 (g) mRNA from BMDMs transfected with specific targeting siRNA or control siRNA. (h) Assay of linoleic acid concentration in the culture supernatants of Mtb-infected BMDMs transfected with Abcg1 specific siRNA or control siRNA for 24 h. (i) Assay of linoleic acid concentration in the culture supernatants of Mtb-infected BMDMs transfected with specific siRNA targeting Fabp4, Fabp5, Fatp1, Fatp2, Abca1, Obp2a, or control siRNA for 24 h. (j-m) qPCR analysis of Fabp4 (j), Fabp5 (k), Fatp1 (l) and Fatp2 (m) mRNA from BMDMs infected with Mtb H37Rv or H37RvΔRv1272c strains for 24 h. (n) Schematic representation of mice experimental design for Fig. 1n–p. C57BL/6 mice were aerosol-infected with approximately 200 c.f.u. per mouse of indicated Mtb strains and treated with or without linoleic acid. (o, p) C57BL/6 mice were aerosol-infected with approximately 200 c.f.u. per mouse of indicated Mtb strains and treated with or without linoleic acid. Linoleic acid concentrations in lung homogenate supernatants (o) and serum (p) were analyzed at 4 weeks post infection. Data in a-m, o, p represent one experiment with three independent biological replicates (n = 3); mean ± s.e.m. Two-tailed unpaired Student’s t-tests (a-g, j-m, o, p) and Two-way ANOVA with Tukey’s multiple comparisons test (h, i) were used for statistical analyses.
