Extended Data Fig. 4: B. fragilis and B. salyersiae produce aGCs and induce NKT cell activation.
From: Human gut bacteria produce structurally related monoglycolipids with contrasting immune functions
a) A MS/MS mirror plot of B. fragilis and B. salyersiae C34:0 aGC species showed an exact match. b) All B. fragilis and B. salyersiae strains, including type strains and clinical isolates, synthesize aGC, in contrast, no tested B. nordi, P. gordornii, and P. copri strains produce aGCs. c) Only aGC-producing bacterial lipid extract can elicit NKT cell activation. NKT cell hybridomas (24.7) were incubated with CD1d-lipid complex and IL-2 secretion was measured by ELISA. Each dot indicates a biologically independent replicate (n = 4 per group). Bars and error bars depict the mean ± s.e.m. Data are representative of three independent experiments showing consistent trends. Statistical analysis was performed using one-way ANOVA. d) Symbiotic Bacteroidales species did not produce aGlcACers, whereas Sphingomonas species served as positive controls and produced aGlcACers.
