Extended Data Fig. 5: Model of TgVIP1 interaction at the PVM with host VAPs on the ER. | Nature Microbiology

Extended Data Fig. 5: Model of TgVIP1 interaction at the PVM with host VAPs on the ER.

From: Toxoplasma gondii VIP1 mediates parasitophorous vacuole–host endoplasmic reticulum interactions to facilitate parasite development

Extended Data Fig. 5: Model of TgVIP1 interaction at the PVM with host VAPs on the ER.The alternative text for this image may have been generated using AI.

EM image shows the close association of the host ER (hER) to the PV membrane. The inset shows a proposed model for TgVIP1 interaction with host VAPs at the ER. The FFAT 2 motif, found to pull-down VAPA and VAPB, is shown interacting with the MSP domain of VAPA and VAPB. FFAT2 only weakly interacts with MOSPD2, based on the pull-down assay (depicted by thinner lines). The accumulation of acidocalcisomes in ∆TgVIP1 parasites in HeLa ∆VAPA∆VAPB and ∆VAPA∆VAPB∆MOSPD2 cells, suggests that TgVIP1 may be involved in Ca2+ transfer/sensing between the host ER and PV, possibly via interaction of host ER stromal interaction molecule 1 (STIM1), a regulator of Ca2+ balance and signaling, and an unknown PVM Ca2+ sensor or channel.

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