Extended Data Fig. 1: MYC knockout rapidly triggers EBV lytic reactivation.
From: Lysine-specific histone demethylase complex restricts Epstein–Barr virus lytic reactivation
(A) Cross-comparison of Avana sgRNA library Day 6 versus Brunello library Day 9 CRISPR screens for host factors that repress EBV reactivation. MYC scored at Day 6 but not Day 9 in the Akata library screen. (B) Immunoblot analysis of WCL from P3HR-1 (left) or Akata (right) cells that expressed control vs Brunello MYC targeting sgRNAs at the indicated days post-transduction. (C) Mean ± SD PM gp350 levels from n = 3 replicates of P3HR-1 (left) or Akata (right) cells expressing control vs MYC sgRNAs as in (B). NS: not significant, *** p < 0.001 by unpaired two-sided Student’s t-test. (D) FACS analysis of Cas9+ EBV+ vs EBV− Akata cells that expressed control or independent screen hit UROD targeting sgRNAs. UROD depletion strongly increase FL4 channel autofluorescence signal. (E) Immunoblot analysis of Akata cells as in (D) that expressed control or UROD targeting sgRNAs. WCL from EBV+ Akata cells ± anti-immunoglobulin (αIgG) cross-linking were used as positive and negative controls. All blots shown are representative of n = 3 replicates.
