Extended Data Fig. 3: LSD1, ZNF217 and CoREST maintain Burkitt B-cell EBV latency.
From: Lysine-specific histone demethylase complex restricts Epstein–Barr virus lytic reactivation
(A) WCL of P3HR-1 cells were subjected to immunoprecipitation using control IgG or anti-LSD1 antibody, followed by immunoblot analysis with 1% input for the indicated proteins. (B) (Left) LSD1, ZNF217 and CoREST relative protein abundances in P3HR-1 ZHT/RHT cells uninduced or induced for lytic replication by 4-HT for the indicated times, or (Right) in EBV-negative (EBV−) versus EBV-positive (EBV+) Akata mock induced or induced for lytic replication by anti-IgG crosslinking for 48 h, using data from Ersing et al.47. Mean ± SD from n = 2 replicates were presented. (C-E) FACS analysis of PM gp350 abundances in P3HR-1 cells expressing control, LSD1 (C), ZNF217 (D) or CoREST (E) targeting sgRNAs. (F) Immunoblot analysis of WCL from EBV super-infected AGS or EBV+ SNU719 gastric carcinoma cells expressing control or ZNF217 sgRNAs. (G) Immunoblot analysis of WCL from EBV+ or EBV- Akata cells expressing control or CoREST sgRNA. Blots are representative of n = 3 independent replicates.
